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KMID : 0861020130280020093
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Korea Journal of Herbology
2013 Volume.28 No. 2 p.93 ~ p.101
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Effects of Baicalein on Neuroinflammation in Lipopolysaccharide-treated Mice
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하경운:Ha Gyung-Woon
김연섭:Kim Youn-Sub
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Abstract
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Objects : Baicalein is a major bioactive flavonoid component of Scutellaria baicalensis Georgi that shows a wide
range of biological activities, including neuroprotections and anti-inflammatory actions. Hence it is a potential therapeutic material for the treatment of neuroinflammation. In this study, we investigated the modulatory effect of baicalein on neuroinflammation.
Method : Pro-inflammatory cytokines (TNF-α, IL-1β and IL-6 mRNA), COX-2 mRNA expression and microglial activation in the brain tissue is induced by systemic lipopolysaccharide (LPS) treatment in C57BL/6 mice. Baicalein was treated orally with 10, 20, and 30 mg/kg 1 hour prior to the LPS (3 ㎎/㎏, i.p.) injection. TNF-α, IL-1β, IL-6 and COX-2 mRNA expression in the brain tissue was measured by the quantitative real-time polymerase chain reaction(PCR) method. Iba1 expression in the brain was measured by western blotting method. Microglia was observed with immunohistochemistry.
Results : Baicalein 30 mg/kg significantly attenuated the expression of TNF-α, IL-1β, IL-6 and COX-2 mRNA in the brain tissue. Baicalein 20 mg/kg significantly attenuated the expression of IL-6 mRNA in the brain tissue. Baicalein 30 mg/kg significantly attenuated the expression of Iba1 protein expression in the brain tissue. Baicalein 30 mg/kg significantly decreased the number and cell size of microglia in the cerebral cortex and hypothalamic region and the area percentage of Iba1-expressed microglia in the hippocampus.
Conclusion : These results demonstrated that baicalein attenuates LPS induced neuroinflammation in the mice via
reduction of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), COX-2 mRNA expression and microglial activation.
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KEYWORD
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Baicalein, TNF-α, IL-1β, IL-6, COX-2, Microglia
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